- 효소촉매 가교반응을 이용한 스프레이형 젤라틴 하이드로젤 창상피복제의 개발
The gelatin hydrogel modified with tyramine (Gel-Tyr) was prepared an enzymatic crosslinking reaction to develop a sprayable wound dressing that is easy to use and hygienic on broad and complicate wound area with biocompatibility, wound healing ability, and comfortable wearing. The substitution rate of Gel-Tyr was calculated through 1H NMR. And the gelation time according to the concentration of gelatin, horseradish peroxidase (HRP) and H2O2, rheological properties, swelling ratio, biodegradation behavior, in vitro cytotoxicity, cell viability was evaluated. The hydrogel with rapid gelation rate and optimum performance as a wound dressing was selected as a sample prepared with a compound concentration of Gel-Tyr 3 wt%, HRP 3.50 units/mL, and H2O2 0.2 μL/mL. In the in vivo full-thickness wound model animal experiment and histological evaluation, the Gel-Tyr hydrogel showed an accelerated wound healing rate and dermal regeneration compared to the control group. It is expected to be used as a sprayable wound dressing in medical treatment, conveniently.