- Putrescine의 존재 하에 중성 pH 조건에서의 가교 히알루론산 하이드로젤의 합성 및 특성
This study is to fabricate hyaluronic acid (HyA) hydrogels under the neutral pH condition in order to minimize the degradation of HyA during the cross-linking reaction. Two kinds of HyA hydrogels (HPDM or HPEN), were prepared with 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methyl-morpholinium chloride (DMTMM) or 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)/N-hydroxysuccinimide (NHS) as cross-linking agents, respectively, in the presence of putrescine as a diamine. Amide bond formation of hydrogels in the presence of putrescine was confirmed by FT-IR analysis. The morphology and rheological property of the hydrogels were studied through a scanning electron microscope and a rheometer, respectively. The swelling property and the degradation behavior of the hydrogels were affected by the two kinds of cross-linking agents and the molar ratio of the putrescine/cross-linking agents. Cytocompatibility of these hydrogels on human dermal fibroblast was confirmed by MTT assay. In conclusion, the cross-linking efficiency and the biocompatibility of HPDM cross-linked by DMTMM were better than those of HPEN cross-linked by EDC/NHS.